multiplex-specific primer design software Search Results


97
New England Biolabs dual indices
Dual Indices, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm multiplex amplicon panel
Multiplex Amplicon Panel, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm access array tm system
Access Array Tm System, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Qiagen dna multiplex pcr kit
Dna Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity ivistm lumina xrms
Ivistm Lumina Xrms, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Sangon Biotech pathogen specific multiplex pcr primers
Pathogen Specific Multiplex Pcr Primers, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Promega pmir-reporter vector
Pmir Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs nebnext index primer
Nebnext Index Primer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs multiplex primers
Multiplex Primers, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp adgre1 mm00802529 m1
(A and B) ASC adipocytes with scramble or linc-ADAIN shRNA were implanted into the flanks of NSG mice and removed 16 weeks later. H&E staining of scramble and linc-ADAIN -KD implants after 16 weeks (scale bar, 60 μm) (A) and size distribution of adipocytes, quantified with Adiposoft in a 300 × 300 μm section (B). (C and D) CD68 (C) and F4/80 (D) IHC staining area quantified. </p/> (E–K) Gene expression of mouse Cd68 (E) and <t>Adgre1</t> (F) and human expression of IL-8 (G), MCP-1 (H), IL-6 (I), KLF5 (J), and PPARγ and CEBPα (K) in the scramble and linc-ADAIN shRNA-expressing explants after 16 weeks or the ASC hTERT adipocytes prior to implantation ( N = 4–7); * p < 0.05, ** p < 0.01, *** p < 0.001 with relation to scramble shRNA explant; ## p < 0.01 with relation to linc-ADAIN shRNA explant; $ $ $ p < 0.01 with reslation to scramble shRNA adipocytes by two-way ANOVA. Data are presented as the mean ± SEM.
Gene Exp Adgre1 Mm00802529 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
iRepertoire Inc highly specific multiplex primers
(A and B) ASC adipocytes with scramble or linc-ADAIN shRNA were implanted into the flanks of NSG mice and removed 16 weeks later. H&E staining of scramble and linc-ADAIN -KD implants after 16 weeks (scale bar, 60 μm) (A) and size distribution of adipocytes, quantified with Adiposoft in a 300 × 300 μm section (B). (C and D) CD68 (C) and F4/80 (D) IHC staining area quantified. </p/> (E–K) Gene expression of mouse Cd68 (E) and <t>Adgre1</t> (F) and human expression of IL-8 (G), MCP-1 (H), IL-6 (I), KLF5 (J), and PPARγ and CEBPα (K) in the scramble and linc-ADAIN shRNA-expressing explants after 16 weeks or the ASC hTERT adipocytes prior to implantation ( N = 4–7); * p < 0.05, ** p < 0.01, *** p < 0.001 with relation to scramble shRNA explant; ## p < 0.01 with relation to linc-ADAIN shRNA explant; $ $ $ p < 0.01 with reslation to scramble shRNA adipocytes by two-way ANOVA. Data are presented as the mean ± SEM.
Highly Specific Multiplex Primers, supplied by iRepertoire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A and B) ASC adipocytes with scramble or linc-ADAIN shRNA were implanted into the flanks of NSG mice and removed 16 weeks later. H&E staining of scramble and linc-ADAIN -KD implants after 16 weeks (scale bar, 60 μm) (A) and size distribution of adipocytes, quantified with Adiposoft in a 300 × 300 μm section (B). (C and D) CD68 (C) and F4/80 (D) IHC staining area quantified. </p/> (E–K) Gene expression of mouse Cd68 (E) and Adgre1 (F) and human expression of IL-8 (G), MCP-1 (H), IL-6 (I), KLF5 (J), and PPARγ and CEBPα (K) in the scramble and linc-ADAIN shRNA-expressing explants after 16 weeks or the ASC hTERT adipocytes prior to implantation ( N = 4–7); * p < 0.05, ** p < 0.01, *** p < 0.001 with relation to scramble shRNA explant; ## p < 0.01 with relation to linc-ADAIN shRNA explant; $ $ $ p < 0.01 with reslation to scramble shRNA adipocytes by two-way ANOVA. Data are presented as the mean ± SEM.

Journal: Cell reports

Article Title: linc-ADAIN , a human adipose lincRNA, regulates adipogenesis by modulating KLF5 and IL-8 mRNA stability

doi: 10.1016/j.celrep.2024.114240

Figure Lengend Snippet: (A and B) ASC adipocytes with scramble or linc-ADAIN shRNA were implanted into the flanks of NSG mice and removed 16 weeks later. H&E staining of scramble and linc-ADAIN -KD implants after 16 weeks (scale bar, 60 μm) (A) and size distribution of adipocytes, quantified with Adiposoft in a 300 × 300 μm section (B). (C and D) CD68 (C) and F4/80 (D) IHC staining area quantified.

(E–K) Gene expression of mouse Cd68 (E) and Adgre1 (F) and human expression of IL-8 (G), MCP-1 (H), IL-6 (I), KLF5 (J), and PPARγ and CEBPα (K) in the scramble and linc-ADAIN shRNA-expressing explants after 16 weeks or the ASC hTERT adipocytes prior to implantation ( N = 4–7); * p < 0.05, ** p < 0.01, *** p < 0.001 with relation to scramble shRNA explant; ## p < 0.01 with relation to linc-ADAIN shRNA explant; $ $ $ p < 0.01 with reslation to scramble shRNA adipocytes by two-way ANOVA. Data are presented as the mean ± SEM.

Article Snippet: Adipose Explant macrophage gene expression was assessed using mouse specific TaqMan primers (ThermoFisher, Mouse Adgre1 Mm00802529_m1, Cd68 Mm03047343) and TaqMan Gene Expression Master Mix (Applied Biosystems).

Techniques: shRNA, Staining, Immunohistochemistry, Gene Expression, Expressing

Journal: Cell reports

Article Title: linc-ADAIN , a human adipose lincRNA, regulates adipogenesis by modulating KLF5 and IL-8 mRNA stability

doi: 10.1016/j.celrep.2024.114240

Figure Lengend Snippet:

Article Snippet: Adipose Explant macrophage gene expression was assessed using mouse specific TaqMan primers (ThermoFisher, Mouse Adgre1 Mm00802529_m1, Cd68 Mm03047343) and TaqMan Gene Expression Master Mix (Applied Biosystems).

Techniques: Virus, Plasmid Preparation, Recombinant, Gene Expression, shRNA, Mass Spectrometry, Negative Control, Software, Modification, Magnetic Beads, RNAscope, Multiplex Assay, Western Blot